Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB

Dr. Markus von Nickisch-Rosenegk

heads the research group Technical Molecular Biology

 

© Fraunhofer IZI-BB

Scientific focus and expertise:

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  • Kwesiga, G., Kelling, A., Kersting, S., Sperlich, E., Nickisch-Rosenegk, M. von, & Schmidt, B. (2020). Total Syntheses of Prenylated Isoflavones from Erythrina sacleuxii and Their Antibacterial Activity: 5-Deoxy-3'-prenylbiochanin A and Erysubin F. Journal of Natural Products, 83(11), 3445–3453. https://doi.org/10.1021/acs.jnatprod.0c00932
  • Kersting, S., Rausch, V., Bier, F. F., & Nickisch-Rosenegk, M. von (2018). A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia. Analytical Biochemistry, 550, 54–60. https://doi.org/10.1016/j.ab.2018.04.014
  • Connor, D. O., Danckert, L., Hoppe, S., Bier, F. F., & Nickisch-Rosenegk, M. von (2017). Epitope determination of immunogenic proteins of Neisseria gonorrhoeae. PloS One, 12(7), e0180962. https://doi.org/10.1371/journal.pone.0180962
  • Connor, D. O., Zantow, J., Hust, M., Bier, F. F., & Nickisch-Rosenegk, M. von (2016). Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display. PloS One, 11(2), e0148986. https://doi.org/10.1371/journal.pone.0148986
  • Jeenduang, N., Porntadavity, S., Nickisch-Rosenegk, M. von, Bier, F. F., & Promptmas, C. (2015). Two-dye based arrayed primer extension for simultaneous multigene detection in lipid metabolism. Clinica Chimica Acta; International Journal of Clinical Chemistry, 442, 36–43. https://doi.org/10.1016/j.cca.2015.01.005
  • Hoppe, S., Bier, F. F., & Nickisch-Rosenegk, M. von (2014). Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PloS One, 9(10), e110703. https://doi.org/10.1371/journal.pone.0110703
  • Danckert, L., Hoppe, S., Bier, F. F., & Nickisch-Rosenegk, M. von (2014). Rapid identification of novel antigens of Salmonella Enteritidis by microarray-based immunoscreening. Mikrochimica Acta, 181(13-14), 1707–1714. https://doi.org/10.1007/s00604-014-1197-6
  • Rapsch, K., Bier, F. F., & Nickisch-Rosenegk, M. von (2014). Rational design of artificial β-strand-forming antimicrobial peptides with biocompatible properties. Molecular Pharmaceutics, 11(10), 3492–3502. https://doi.org/10.1021/mp500271c
  • Kersting, S., Rausch, V., Bier, F. F., & Nickisch-Rosenegk, M. von (2014). Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens. Mikrochimica Acta, 181(13-14), 1715–1723. https://doi.org/10.1007/s00604-014-1198-5
  • Kersting, S., Rausch, V., Bier, F. F., & Nickisch-Rosenegk, M. von (2014). Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria Journal, 13, 99. https://doi.org/10.1186/1475-2875-13-99
  • Rapsch, K., Bier, F. F., Tadros, M., & Nickisch-Rosenegk, M. von (2014). Identification of antimicrobial peptides and immobilization strategy suitable for a covalent surface coating with biocompatible properties. Bioconjugate Chemistry, 25(2), 308–319. https://doi.org/10.1021/bc4004469
  • Herbel, S., Nickisch-Rosenegk, M. von, Kuhn, M., Murugaiyan, J., Wieler, L., & Guenther, S. (2014). Specific TaqMan Probes for the Identification and Quantification of Lactobacilli in Pharmaceuticals. Journal of Probiotics & Health, 2.
  • Hoppe, S., Bier, F. F., & Nickisch-Rosenegk, M. von (2013). Rapid identification of novel immunodominant proteins and characterization of a specific linear epitope of Campylobacter jejuni. PloS One, 8(5), e65837. https://doi.org/10.1371/journal.pone.0065837
  • Herbel, S. R., Lauzat, B., Nickisch-Rosenegk, M. von, Kuhn, M., Murugaiyan, J., Wieler, L. H., & Guenther, S. (2013). Species-specific quantification of probiotic lactobacilli in yoghurt by quantitative real-time PCR. Journal of Applied Microbiology, 115(6), 1402–1410. https://doi.org/10.1111/jam.12341
  • Nickisch-Rosenegk, M. von, Teschke, T., & Bier, F. F. (2012). Construction of an artificial cell membrane anchor using DARC as a fitting for artificial extracellular functionalities of eukaryotic cells. Journal of Nanobiotechnology, 10, 1. https://doi.org/10.1186/1477-3155-10-1
  • Hoppe, S., Bier, F. F., & Nickisch-Rosenegk, M. von (2012). Microarray-based method for screening of immunogenic proteins from bacteria. Journal of Nanobiotechnology, 10, 12. https://doi.org/10.1186/1477-3155-10-12
  • Andresen, D., Nickisch-Rosenegk, M. von, & Bier, F. F. (2009). Helicase-dependent amplification: Use in OnChip amplification and potential for point-of-care diagnostics. Expert Review of Molecular Diagnostics, 9(7), 645–650. https://doi.org/10.1586/erm.09.46
  • Andresen, D., Nickisch-Rosenegk, M. von, & Bier, F. F. (2009). Helicase dependent OnChip-amplification and its use in multiplex pathogen detection. Clinica Chimica Acta; International Journal of Clinical Chemistry, 403(1-2), 244–248. https://doi.org/10.1016/j.cca.2009.03.021
  • Guenther, S., Nöckler, K., Nickisch-Rosenegk, M. von, Landgraf, M., Ewers, C., Wieler, L. H., & Schierack, P. (2008). Detection of Trichinella spiralis, T. Britovi and T. Pseudospiralis in muscle tissue with real-time PCR. Journal of Microbiological Methods, 75(2), 287–292. https://doi.org/10.1016/j.mimet.2008.06.019
  • Bier, F. F., Nickisch-Rosenegk, M. von, Ehrentreich-Förster, E., Reiss, E., Henkel, J., Strehlow, R., & Andresen, D. (2008). Dna microarrays. Advances in Biochemical Engineering/biotechnology, 109, 433–453. https://doi.org/10.1007/10_2007_087
  • Nickisch-Rosenegk, M. von, Marschan, X., Andresen, D., & Bier, F. F. (2008). Reverse transcription-polymerase chain reaction on a microarray: The integrating concept of "active arrays". Analytical and Bioanalytical Chemistry, 391(5), 1671–1678. https://doi.org/10.1007/s00216-008-2154-1
  • Guenther, S., Nöckler, K., von Nickisch-Rosenegk, M., Landgraf, M., Ewers, C., Wieler, L. H., & Schierack, P. (2008). Detection of Trichinella spiralis, T. britovi and T. pseudospiralis in muscle tissue with real-time PCR. Journal of microbiological methods, 75(2), 287-292.
  • Guenther, S., Landgraf, M., v. Nickisch-Rosenegk, M., Kuhn, M., Bier, F., Wieler, L. H., & Schierack, P. (2008, September). Development of a fast and parallel detection system for zoonotic pathogens from swine in the whole food chain. In INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY (Vol. 298, pp. 60-60). OFFICE JENA, PO BOX 100537, 07705 JENA, GERMANY: ELSEVIER GMBH, URBAN & FISCHER VERLAG.
  • Nickisch-Rosenegk, M. von, Ehrentreich-Forster, E., Strehlow, R., Christmann, A., & Bier, F. F. (2005). Chemically synthesized zinc finger molecules as nano-addressable probes for double-stranded DNAs. Journal of Nanobiotechnology, 3, 5. https://doi.org/10.1186/1477-3155-3-5
  • Nickisch-Rosenegk, M. von, Marschan, X., & Bier, F. F. (2005). Integration of the One-Step-RT-PCR by On-Chip-PCR on oligonucleotide-based microarrays. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_000784
  • Teschke, T., Nickisch-Rosenegk, M. von, & Bier, F. F. (2005). A plasma membrane-anchor for extracellularly expressed proteins. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_001336
  • Nickisch-Rosenegk, M. von, Marschan, X., Andresen, D., Abraham, A., Heise, C., & Bier, F. F. (2005). On-chip PCR amplification of very long templates using immobilized primers on glassy surfaces. Biosensors and Bioelectronics, 20(8), 1491–1498. https://doi.org/10.1016/j.bios.2004.07.007
  • Andresen, D., Nickisch-Rosenegk, M. v., Kuhn, M., & Bier, F. F. (2005). Development of a DNA-Chip for detection of pathogens in poultry. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_001414
  • Goris, T., Nickisch-Rosenegk, M. von, & Bier, F. F. (2005). Construction of cDNA templates as a basis for homologous and linear amplification of RNA. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_001342
  • Steffen, J., Nickisch-Rosenegk, M. von, & Bier, F. F. (2005). In vitro transcription of a whole gene on a surface-coupled template. Lab on a Chip, 5(6), 665–668. https://doi.org/10.1039/b501091h
  • Steffen, J., Nickisch-Rosenegk, M. von, & Bier, F. F. (2005). In vitro transcription of a whole gene on a surface-coupled template. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_001332
  • Reiß, E., Hölzel, R., Nickisch-Rosenegk, M. von, & Bier, F. F. (2005). Directed single-stranded DNA-synthesis via rolling circle amplification in an alternating electric field. GBM Annual Fall Meeting Berlin/Potsdam 2005, 2005(Fall). https://doi.org/10.1240/sav_gbm_2005_h_001337

RAPSCH, KARSTEN; NICKISCH-ROSENEGK, MARKUS VON: Synthetic artificial peptides having an anti-microbial effect. DE 102014101663 (A1) 

NICKISCH-ROSENEGK, MARKUS VON: Gentechnisch modifizierte Bakteriophagen, insbesondere zur Bekämpfung von pathogenen Prokaryonten bzw. ihrer pathologischen Wirkung, sowie deren Verwendung und Herstellung. DE102007003148 (A1)

  • Prokaryotic cDNA Expression Libraries
  • Phage Display
  • Microbial knock-out mutants
  • Antibody-mediated FRET detection > visual evaluation
  • ATP/NADH-mediated energy cascades > visual evaluation
  • Dipstick-integrated isothermal amplification > visual evaluation
  • Specific microbial detection by differential refractive index measurement > visual evaluation