Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB

Dr. Michael Kirschbaum

Dr. Michael Kirschbaum studied biology with a focus on neurobiology, physics and biochemistry at the University of Tübingen. After graduating in 2005, he joined the Lab-On-Chip Technologies group at the Fraunhofer Institute in Potsdam, where he received his PhD in 2009 and has headed the Microfluidic Cell Processing & Cell Analytics group since 2011.

 

© Fraunhofer IZI-BB

Scientific focus and expertise:

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Michael Kirschbaum - ORCID: 0000-0003-3686-8349
  • Gerling T, Godino N, Pfisterer F, Hupf N, Kirschbaum M. (2023) High-precision, low-complexity, high-resolution microscopy-based cell sorting. Lab on a chip. doi: 10.1039/D3LC00242J.
  • Pfisterer F, Godino N, Gerling T, Kirschbaum M. (2023) Continuous microfluidic flow-through protocol for selective and image-activated electroporation of single cells. RSC advances. doi: 10.1039/D3RA03100D.
  • Dudaie M, Nissim N, Barnea I, Gerling T, Duschl C, Kirschbaum M, Shaked NT. (2020) Label-free discrimination and selection of cancer cells from blood during flow using holography-induced dielectrophoresis. Journal of biophotonics. doi: 10.1002/jbio.202000151.
  • Behm LVJ, Gerike S, Grauel MK, Uhlig K, Pfisterer F, Baumann W, Bier FF, Duschl C, Kirschbaum M. (2019) Micropatterned Thermoresponsive Cell Culture Substrates for Dynamically Controlling Neurite Outgrowth and Neuronal Connectivity in Vitro. ACS applied bio materials. doi: 10.1021/acsabm.9b00246.
  • Godino N, Pfisterer F, Gerling T, Guernth-Marschner C, Duschl C, Kirschbaum M. (2019) Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis. Lab on a chip. doi: 10.1039/C9LC00800D.
  • Behm LVJ, Schlenther I, Petrausch M, Jorde F, Godino N, Pfisterer F, Duschl C, Kirschbaum M. (2018) A simple approach for the precise measurement of surface temperature distributions on the microscale under dry and liquid conditions based on thin Rhodamine B films. Sensors and Actuators B: Chemical. doi: 10.1016/j.snb.2017.09.001.
  • Habaza M, Kirschbaum M, Guernth-Marschner C, Dardikman G, Barnea I, Korenstein R, Duschl C, Shaked NT. Rapid 3D Refractive-Index Imaging of Live Cells in Suspension without Labeling Using Dielectrophoretic Cell Rotation. Adv. Sci. (2017), 4, 1600205
  • Kirschbaum M, Jaeger MS, Duschl C. (2015) Measurement of surface-mediated Ca2+ transients on the single-cell level in a microfluidic lab-on-a-chip environment. Methods in molecular biology (Clifton, N.J.). doi: 10.1007/978-1-4939-2336-6_17.
  • Schreml S, Meier RJ, Kirschbaum M, Kong SC, Gehmert S, Felthaus O, Küchler S, Sharpe JR, Wöltje K, Weiß KT, Albert M, Seidl U, Schröder J, Morsczeck C, Prantl L, Duschl C, Pedersen SF, Gosau M, Berneburg M, Wolfbeis OS, Landthaler M, Babilas P. (2014) Luminescent dual sensors reveal extracellular pH-gradients and hypoxia on chronic wounds that disrupt epidermal repair. Theranostics. doi: 10.7150/thno.9052.
  • Guernth-Marschner C, Kirschbaum M, Jaeger MS, Duschl C. Electrofusion of cells in microdevices. Cell News. (2013), 39(3), 14-18.
  • Kirschbaum M, Gürnth-Marschner CR, Cherré S, de Pablo Peña A, Jäger MS, Kroczek RA, Schnelle T, Müller T, Duschl C. Highly controlled single-cell electrofusion in dielectrophoretic field cages. Lab on a Chip. (2012), 12, S. 443-450.
  • Guido I, Xiong C, Jaeger MS, Duschl C. Microfluidic system for cell mechanics analysis through dielectrophoresis. Microelectron Eng. (2012), 97:379-382
  • Boettcher M, Schmidt S, Latz A, Jaeger MS, Stuke M, Duschl C. Filtration at the microfluidic level: enrichment of nanoparticles by tunable filters. J Phys Condens Mat. (2011), 23, 324101
  • Guido I, Jaeger MS, Duschl C. Dielectrophoretic stretching of cells allows for characterization of their mechanical properties. Eur Biophys J. (2011), 40:281-288.
  • Guido I, Jaeger MS, Duschl C. Influence of medium consumption on cell elasticity. Cytotechnology. (2010), 62, 257-263.
  • Kirschbaum M, Jaeger MS, Duschl C. Correlating short-term Ca2+ responses with long-term protein expression after activation of single T cells. Lab Chip. (2009), 9, 3517-3525.
  • Kirschbaum M, Jaeger MS, Schenkel T, Breinig T, Meyerhans A, Duschl C. T cell activation on a single-cell level in dielectrophoresis-based microfluidic devices. J Chromatogr A. (2008), 1202, 83–89.
  • Böttcher M, Jäger MS, Kirschbaum M, Müller T, Schnelle T, Duschl C. Gravitation-driven stress-reduced cell handling. Anal Bioanal Chem. (2008), 390, 857-863.
  • Storn V, Kirschbaum M, Schlosshauer B, Mack AF, Fricke C. Electrical stimulation-induced release of beta-endorphin from genetically modified neuro-2a cells. Cell Transplant. (2008), 17(5):543-8
  • Jaeger MS, Uhlig K, Schnelle T, Mueller T. Contact-free single-cell cultivation by negative dielectrophoresis. J Phys D Appl Phys. (2008), 41:175502.
  • Jaeger MS, Mueller T, Schnelle T. Thermometry in dielectrophoresis chips for contact-free cell handling. J Phys D Appl Phys. (2007), 40:95–105
  • Böttcher M, Jäger MS, Riegger L, Ducrée J, Zengerle R, Duschl C. Lab-on-chip-based cell separation by combining dielectrophoresis and centrifugation. BRL. (2006), 1(4):443-451.

Cell cultivation, cell analysis, microscopy, microfluidics, liquid handling, microfabrication:

  • Design and construction of chip-based microsystems
  • Precise and gentle handling of individual cells and microparticles in closed microfluidic systems
  • Lossless processing of small, valuable cell samples
  • Image-based flow cytometry and image-based cell sorting
  • Blood cell separation
  • In vitro test procedure for dynamic blood compatibility testing of (coronary) stents